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细胞培养过程中如果操作不当或者条件控制不合适见容易受到化学或者生物因素的污染,常见的污染源有: 1、细菌污染   ...

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规格参数:目录编号规格形状适用器皿表面处理灭菌包装规格WHB-6-CSφ24mm圆形6孔板、35mm皿TC处理伽马...

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细胞培养板依底部形状的不同可分为平底和圆底(U型和V型);培养孔的孔数有6、12、24、48、96、384、153...

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大部分动物细胞需要贴附在一个固相界面上才能生长。而只有亲水的固相界面,细胞才能贴附。 最早的细胞培养器皿是由玻璃制...

< class='newsTypePicList_four newsListPicList2'>技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='65' newsName='石蜡切片免疫组化实验-卵白素-生物素-过氧化物酶复合物(ABC法)' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/09/16 < id='lineBody65' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > 石蜡切片免疫组化实验-卵白素-生物素-过氧化物酶复合物(ABC法) <>

实验材料 蜡块切片试剂、试剂盒多聚甲醛 蒸馏水 蔗糖 过氧化氢 甲醇 PBS 酒精 KPBS 牛血清白蛋白 叠氮纳...

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实验方法原理根据聚合酶技术把辣根过氧化物酶抗鼠或/和抗兔IgG分子结合在多聚酶上形成多聚物分子,其与待检的抗原特异...

< class='newsTypePicList_four newsListPicList2'>试验方法技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='63' newsName='蛋白质免疫印迹(Western Blot)Western印迹法' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/09/14 < id='lineBody63' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > 蛋白质免疫印迹(Western Blot)Western印迹法 <>

实验材料蛋白质样品试剂、试剂盒裂解液 PBS G 250考马斯亮蓝溶液 NaCl SDS上样缓冲液 电泳缓冲液 转...

< class='newsTypePicList_four newsListPicList2'>技术指导试验方法 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='59' newsName='Hoechst染色' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/08/31 < id='lineBody59' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > Hoechst染色 <>

一、贴壁细胞1.  取普通洁净盖玻片于70%乙醇中浸泡5分钟或更长时间,无菌超净台内吹干或用细胞培养PBS或0.9...

< class='newsTypePicList_four newsListPicList2'>技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='58' newsName='MTT检测细胞活性的操作方法' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/08/31 < id='lineBody58' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > MTT检测细胞活性的操作方法 <>

一、MTT是什么 MTT是一种粉末状化学试剂,全称为3-(4,5)-dimethylthiahiazo (-z-y...

< class='newsTypePicList_four newsListPicList2'>技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='56' newsName='细胞凋亡实验步骤及注意事项' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/08/31 < id='lineBody56' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > 细胞凋亡实验步骤及注意事项 <>

一、实验目的1、掌屋凋亡细胞的形态特征   2、学会用荧光探针对细胞进行双标记来检测正常活细胞、凋亡细胞和坏死细胞...

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这里想明确两个概念,一个是Transwell,另一个是肿瘤细胞侵袭模型。1.Transwell关于Transwel...

< class='newsTypePicList_four newsListPicList2'>技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='52' newsName='细胞转染方法与原则' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/08/20 < id='lineBody52' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > 细胞转染方法与原则 <>

  细胞转染在研究基因功能、调控基因表达、突变分析和蛋白质生产等方面的应用越来越广泛。细胞转染常用的主要有三种方法...

< class='newsTypePicList_four newsListPicList2'>技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='43' newsName='细胞培养的基本设备和器材' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/08/10 < id='lineBody43' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > 细胞培养的基本设备和器材 <>

一、基本设备      1、CO2培养箱或/和普通隔水式培养箱  2、超净工作台  3、无菌室  4、普通普微镜 ...

< class='newsTypePicList_four newsListPicList2'>技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='41' newsName='细胞污染的思考和自己的体会' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/08/10 < id='lineBody41' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > 细胞污染的思考和自己的体会 <>

细胞污染的思考本人最近老遭到细胞的污染,前期的实验都在这一关被卡住,很是郁闷,需要追其原因,避免再次发生,由此小结...

< class='newsTypePicList_four newsListPicList2'>技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='40' newsName='细胞培养板的选择与常见问题解答' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/08/10 < id='lineBody40' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > 细胞培养板的选择与常见问题解答 <>

细胞培养板依底部形状的不同可分为平底和圆底(U型和V型);培养孔的孔数有6、12、24、48、96、384、153...

< class='newsTypePicList_four newsListPicList2'>技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='39' newsName='细胞冻存与复苏技术' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/08/10 < id='lineBody39' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > 细胞冻存与复苏技术 <>

概述细胞培养技术自1907年开创以来,历经一个世纪现已成为自然科学领域不可缺少的研究方法之一。在细胞培养技术广泛用...

< class='newsTypePicList_four newsListPicList2'>技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='38' newsName='细胞培养污染的思考' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/08/10 < id='lineBody38' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > 细胞培养污染的思考 <>

污染是细胞培养技术中面临的主要问题。由每一种细胞有其独特的培养体系,因此污染造成后果也不尽相同。某些污染的发生往往...

< class='newsTypePicList_four newsListPicList2'>技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='37' newsName='细胞培养污染拯救及预防方法' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/08/10 < id='lineBody37' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > 细胞培养污染拯救及预防方法 <>

细胞培养污染拯救及预防方法§ 污染是细胞培养的大敌。预防和避免污染是细胞培养成功的关键之一。§ 一开始就要十分重视...

< class='newsTypePicList_four newsListPicList2'>技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='36' newsName='细胞培养知识和技术' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/08/09 < id='lineBody36' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > 细胞培养知识和技术 <>

1、细胞(组织)培养年鉴1878 Claude Bernard:证明一个器官的生理系统在个体死亡以后仍然可以人工维...

< class='newsTypePicList_four newsListPicList2'>技术指导 < class='J_separatorLine separatorLine g_separator '> < topClassName='top1' topSwitch='on' newsId='35' newsName='细胞培养常见问题及回答' class='J_newsListLine line g_item fk-newsLineHeight J_nHover '>< class='J_newsCalendar newsCalendar newsListCalendar2' style='vertical-align:top;'> 2015/08/09 < id='lineBody35' class='J_lineBody lineBody newsListLineBody2'>< class='newsCircleOuter'>< class='newsCircle'> < class='newsTitlePanel'> < class='J_newsTitle newsTitle'> < class='J_newsListTopFlag ' > 细胞培养常见问题及回答 <>

1、如何选用培养基? 培养某一类型细胞没有固定的培养条件。在MEM中培养的细胞,很可能在DMEM或M199中同样很...

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