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< style=width:470px;height:470px; alt='细胞爬片 Cover Glasses' src='http://6221156.s21i.faiusr.com/2/ABUIABACGAAg34ah9AUo4KvMhAYwrgQ4rgQ!500x500.jpg' >
< class='J_productOpera productOpera fk-productOperaStyle'>< id='pdCollection' class='pdCollection'>< class=' faisco-icons-start2 pdCollectIcon f-collectIcon text'>< class=' collection text'>收藏< id='pdShareDiv' class='pdShareDiv'>< id='pdShare' class='pdShare'>< class=' faisco-icons-share12 pdShareIcon text'>< class='share text '>分享< class='shareInfo shareInfo_J'>< class='m_shareCtrl'>< id='pdSerOnlineDiv' class='pdSerOnlineDiv'>< id='pdSerOnline' class='pdSerOnline'>< class='faisco-icons-message5 pdKefuIcon text '>< class='serOnline text ' _title='在线客服'>在线客服< class='serOnlineInfo J_serOnlineInfo'>< class='serOnline-service'>< serviceType='0' class='serOnline-list-h lineH-21 serOnline-list '> 客服一< serviceType='4' class='serOnline-list-h lineH-21 serOnline-list lastData'> 客服一< class='serOnlineClearFloat'>< class='fk-hasTitlePageStyle' id='prevAndNextDiv' style='' > < id='singleNewspagenation14' > < class='pagenation2 f-pagePre'>上一个:防脱载玻片 Adhesion Microscope Slides < class='pagenation2 f-pageNext'>下一个:细胞培养瓶 Cell Culture Flasks
< class='pdLayoutR pdLayoutR_J'>< class='J_productTitle title g_minor'>细胞爬片 Cover Glasses  < class='productPropDiv J_productProp'>< class='pd_propTable '>
目录价格:
300.00~5000.00
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产品材质: 高透盖玻片
灭菌方式: 伽马射线灭菌
产品品牌: WHB
货    期: 现货
产品货号:
产品规格:
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< src="http://6221156.s21i.faiusr.com/4/ABUIABAEGAAgiZHS8wUozLbnuQcwJjgc.png" width="38" height="28" alt="图标1.png" /> 细胞爬片 Cover Glasses


< src="http://6221156.s21i.faiusr.com/2/ABUIABACGAAg2ZPS8wUorp_mogMwigE4Kw.jpg" width="138" height="43" alt="规格.jpg" />


分类货号品名 尺寸规格
目录价

圆形爬片

WHB-6-CS
TC处理6孔板圆形细胞爬片,已灭菌Φ24mm100片/盒300.00
WHB-12-CSTC处理12孔板圆形细胞爬片,已灭菌Φ20mm100片/盒300.00
WHB-24-CSTC处理24孔板圆形细胞爬片,已灭菌Φ14mm100片/盒300.00
WHB-48-CSTC处理48孔板圆形细胞爬片,已灭菌Φ8mm100片/盒400.00
WHB-96-CSTC处理96孔板圆形细胞爬片,已灭菌Φ3mm100片/盒500.00

方形爬片

WHB-6-CS-24TC处理6孔板方形细胞爬片,已灭菌□24*24mm100片/盒300.00
WHB-12-CS-15TC处理12孔板方形细胞爬片,已灭菌□15*15mm100片/盒300.00
WHB-24-CS-10TC处理24孔板方形细胞爬片,已灭菌□10*10mm100片/盒300.00
WHB-96-CS-4TC处理96孔板方形细胞爬片,已灭菌□4*4mm100片/盒500.00

多聚赖氨酸

WHB-6-CS-LC多聚赖氨酸包被6孔细胞爬片,已灭菌Φ24mm100片/盒670.00
WHB-12-CS-LC多聚赖氨酸包被12孔细胞爬片,已灭菌Φ20mm100片/盒670.00
WHB-24-CS-LC多聚赖氨酸包被24孔细胞爬片,已灭菌Φ14mm100片/盒670.00
WHB-48-CS-LC多聚赖氨酸包被48孔细胞爬片,已灭菌Φ8mm100片/盒880.00



< src="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgiJzS8wUo8ImbzAMwigE4Kw.jpg" width="138" height="43" alt="材质.jpg" />


< src="http://6221156.s21i.faiusr.com/4/ABUIABAEGAAgiZHS8wUozLbnuQcwJjgc.png" width="38" height="28" alt="图标1.png" /> 高透耐酸碱盖玻片,厚度:0.17mm


< src="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgv57S8wUorreoggEwigE4Kw.jpg" width="138" height="43" alt="特点.jpg" />


       WHB一步法细胞爬片采用高端的玻片制作,厚度为0.15mm,有圆形和方形。已处理,已灭菌,拆开即用。采用先进的玻片表面处理技术(TC处理→超强吸附)可促进细胞在玻片上贴壁生长,细胞贴壁牢固。即使在后期免疫组化、免疫荧光、原位杂交处理过程中也不易脱片,避免传统方法中从培养瓶转移到载玻片上的损伤。使用一步法细胞爬片不用预处理,避免在后期处理中细胞容易脱片等种种问题。

  • 爬片常用的规格:圆形(φ24mm,φ20mm,φ14mm,φ8mm,φ3mm) 方形(24*24mm,15*15mm,10*10mm,4*4mm)。

  • WHB细胞爬片有TC处理和多聚赖氨酸包被两种,双面均可使用,避免了实验误差。

  • TC处理适用于贴壁较好的细胞,多聚赖氨酸包被适用于贴壁不好的细胞比如神经细胞和转染细胞。

  • 爬片高强度耐酸碱,特殊实验浸泡一个星期均不会碎片。

  • 一步法细胞爬片只需打开包装即可使用,节省成本,节省实验员的试验时间。

  • 经过伽马射线灭菌,无DNA酶,无RNA酶,无热源。

< src="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgt7Wg9AUogun3zQIwigE4Kw.jpg" width="138" height="43" alt="应用.jpg" />


< src="http://6221156.s21i.faiusr.com/4/ABUIABAEGAAgiZHS8wUozLbnuQcwJjgc.png" width="38" height="28" alt="图标1.png" /> 组织学,免疫组织化学,冰冻切片,细胞涂片,原位杂交等。


< src="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgipOh9AUo2YX1igUwigE4Kw.jpg" width="138" height="43" alt="实验图片.jpg" />


▼不同细胞使用WHB细胞爬片培养下的细胞状态对比图
< src="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgwZSh9AUohvb1xQYwqQE4eQ.jpg" width="169" height="121" alt="实验图片-1.jpg" />< src="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgwZSh9AUo8Mnh6wEwqQE4eQ.jpg" width="169" height="121" alt="实验图片-2.jpg" />< src="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgwpSh9AUozK6vygYwqQE4eQ.jpg" width="169" height="121" alt="实验图片-3.jpg" />
L929细胞24h(1.5x10/ml)5CH0细胞48h(2x10/ml)5HEK293细胞48h(2x10/ml)5


< src="//0ms.faisys.com/image/loading/dot.gif" width="138" height="43" alt="注意事项.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgqpah9AUoooSarwcwigE4Kw.jpg">


1. 在超净台内,使用75%乙醇擦拭外包装铝箔袋。

2. 拆开铝箔袋,取出内置器皿后,用尖镊将内置爬片取出放入所用培养板或培养皿内。

3. 用胰酶消化好细胞,充分吹打,使之成单细胞悬液(注意:这一点很重要,关系到将来爬出来片子的质量)。

4. 种植细胞时,根据玻片的大小,先在每个孔里准备放爬片的位置滴少量培养基,目的是使玻片与培养皿靠培养基的张力粘合到一起,然后放玻片,防止加细胞悬液时玻片漂起,造成双层细胞贴片。整个过程注意无菌操作。

5. 根据自己的需要选择合适的细胞密度种入培养板内即可。

6. 使用细胞爬片时,(比如在6孔板钟放入爬片,6孔板的孔底面积往往比爬片面积多出一部分,加细胞悬液时,常常就是细胞铺满整个孔底,有时,细胞生长边集现象,就是靠近壁边缘细胞密度要高一些,这样处于中央细胞爬片上的细胞数量相对较少),比较好的做法是,将爬片放入孔内后,加细胞悬液时,只滴到爬片上,一直滴到液体布满整个爬片,但又不易溢出爬片边缘,放到培养箱里,等细胞贴壁后,取出,再滴加培养基布满整个孔底,继续培养,这样爬片上的细胞生长的密度就会很集中。

7. 细胞放到爬片上,之后加任何试剂,都应沿培养板板壁少量一点点倒入液体,直到细胞被液体淹没,尽量不要把细胞冲到细胞爬片以外!

8. 作用一定时间后取玻片。取玻片时由于玻片与培养皿底结合较紧,张力较大,一般将注射器针头针尖向背面弄个小钩,这样将爬片轻轻勾起,用小镊子取出就可以了。如果要做诸如24h,48h,72h等这样时间点的实验的话,将所需数量的爬片取出时应用酒精灯火焰烧一下针头和镊子。末取出的可接着继续培养。

9. 细胞爬片,有多层包装,在超净台无菌状态下打开包装,未用完的爬片按照原先包装方式再包装起来即可。

10. 爬片表面带有正电荷,请勿用手触摸,以免效果不佳!


< src="//0ms.faisys.com/image/loading/dot.gif" alt="产品细节.png" style="width:1200px;height:152px;" / data-original="http://6221156.s21i.faiusr.com/4/ABUIABAEGAAgtKDS8wUoirX7jgcwsAk4mAE.png">



< src="//0ms.faisys.com/image/loading/dot.gif" width="390" height="241" alt="爬片7.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgr5ih9AUoxL3ljwIwhgM48QE.jpg">< src="//0ms.faisys.com/image/loading/dot.gif" width="390" height="241" alt="爬片8.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgr5ih9AUovsLl-gIwhgM48QE.jpg">
采用新款避光型吸塑真空包装,防止污染易撕口方便拆装保存
< src="//0ms.faisys.com/image/loading/dot.gif" width="390" height="241" alt="爬片9.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgr5ih9AUo1vmKVDCGAzjxAQ.jpg">< src="//0ms.faisys.com/image/loading/dot.gif" width="390" height="241" alt="爬片10.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgr5ih9AUojtCYoAEwhgM48QE.jpg">
WHB细胞爬片可匹配WHB专用细胞培养皿和培养板使用爬片采用γ射线灭菌,呈淡茶色属于正常现象,放心使用


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< src="//0ms.faisys.com/image/loading/dot.gif" width="403" height="286" alt="爬片WHB-6-CS.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgsZuh9AUo0KrdmwUwkwM4ngI.jpg">< src="//0ms.faisys.com/image/loading/dot.gif" width="403" height="286" alt="爬片WHB-12-cs.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgsZuh9AUowoCq8gQwkwM4ngI.jpg">
WHB-6-CSWHB-12-CS
< src="//0ms.faisys.com/image/loading/dot.gif" width="403" height="286" alt="爬片WHB-24-CS.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgsZuh9AUogNOMlgQwkwM4ngI.jpg">< src="//0ms.faisys.com/image/loading/dot.gif" width="403" height="286" alt="爬片WHB-48-CS.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgs5uh9AUo1q_lpgEwkwM4ngI.jpg">
WHB-24-CSWHB-48-CS
< src="//0ms.faisys.com/image/loading/dot.gif" width="403" height="286" alt="爬片WHB-96-CS.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgs5uh9AUogPygzwUwkwM4ngI.jpg">< src="//0ms.faisys.com/image/loading/dot.gif" width="403" height="286" alt="爬片WHB-6-CS-24.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgsZuh9AUosoma1AYwkwM4ngI.jpg">
WHB-96-CSWHB-6-CS-24
< src="//0ms.faisys.com/image/loading/dot.gif" width="403" height="286" alt="爬片WHB-12-CS-15.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgsZuh9AUo8LSz8gIwkwM4ngI.jpg">< src="//0ms.faisys.com/image/loading/dot.gif" width="403" height="286" alt="爬片WHB-24-CS-10.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAgs5uh9AUoqKuk0AIwkwM4ngI.jpg">
WHB-12-CS-15
WHB-24-CS-10



< src="//0ms.faisys.com/image/loading/dot.gif" width="827" height="463" alt="爬片11.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAggZ_h9AUo5Jvx1gEwuwY4zwM.jpg">


< src="//0ms.faisys.com/image/loading/dot.gif" width="827" height="463" alt="爬片12.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAggZ_h9AUopouH9wIwuwY4zwM.jpg">


< src="//0ms.faisys.com/image/loading/dot.gif" width="827" height="463" alt="爬片14.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAggZ_h9AUo2NKejAEwuwY4zwM.jpg">


< src="//0ms.faisys.com/image/loading/dot.gif" width="827" height="463" alt="爬片13.jpg" / data-original="http://6221156.s21i.faiusr.com/2/ABUIABACGAAggZ_h9AUojK3WtAIwuwY4zwM.jpg">


< id='accumulativeComment' class='fk-accumulativeComment'> < id='saleRecordPanel' class='fk-saleRecordPanel' > < class='saleRecordHeader'>
买家选项信息数量成交时间
< class='saleRecordBody'>
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< id="bottomForms" class="column forms mainForms bottomForms" style='display:none'> < id="containerPlaceholder" class="containerPlaceholder" > < id="containerMiddleCenterBottom" class="containerMiddleCenterBottom"> < id="containerMiddleRight" class="containerMiddleRight"> < class="containerBottom"> < class="left"> < class="center"> < class="right"> < id="fullmeasureBottomForms" class="fullmeasureContainer forms sideForms fullmeasureForms fullmeasureBottomForms" style='display:none'> < class="absBottomTable"> < id="absBottomForms" class="forms sideForms absForms"> < style="position:absolute;"> < id="webFooterTable" class="webFooterTable J_webFooterTable" > < class="fk-moduleZoneWrap"> < id="fk-webFooterZone" class="elemZone elemZoneModule J_moduleZone fk-webFooterZone fk-moduleZone forms sideForms"> < class="fk-moduleZoneBg fk-elemZoneBg J_zoneContentBg elemZoneBg"> < id="webFooter" class="webFooter"> < id='footer' class='footer' >< class='footerTop'>< class='topLeft'>< class='topCenter'>< class='topRight'>< class='footerMiddle'>< class='middleLeft'>< class='middleCenter'>< class='footerContent'>< id = 'footerInfoV1' class='footerInfo'>
< src="//0ms.faisys.com/image/loading/dot.gif" style="width:30px;height:30px;" alt=" " / data-original="http://6221156.s21i.faiusr.com/4/ABUIABAEGAAgw-L4vgUov7SqywEwZDhk!60x60.png">深圳市福田区深南中路3009号福田大厦< src="//0ms.faisys.com/image/loading/dot.gif" style="width:30px;height:30px;" alt=" " / data-original="http://6221156.s21i.faiusr.com/4/ABUIABAEGAAgzvL4vgUokLfZhQUwZDhk!60x60.png">电话:13623588900< src="//0ms.faisys.com/image/loading/dot.gif" style="width:30px;height:30px;" alt=" " / data-original="http://6221156.s21i.faiusr.com/4/ABUIABAEGAAgwfL4vgUos6nIxwcwZDhk!60x60.png">工作时间:8:30~17:30< src="//0ms.faisys.com/image/loading/dot.gif" style="width:35px;height:35px;" alt=" " / data-original="http://6221156.s21i.faiusr.com/4/ABUIABAEGAAgv-L4vgUos6TozwMwZDhk!60x60.png">邮箱:8623379617@qq.com


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< class='footerSupport' id='footerSupport'> 粤ICP备17130624号-1 < class='middleRight'>< class='footerBottom'>< class='bottomLeft'>< class='bottomCenter'>< class='bottomRight'> < class="clearfloat"> < class="floatLeftTop manageTranTop" style='top:35px' > < id="floatLeftTopForms" class="forms sideForms floatForms"> < class="floatRightTop manageTranTop" style='top:35px' > < id="floatRightTopForms" class="forms sideForms floatForms"> < id='module1343' _indexClass='formIndex1' _moduleType='1' _modulestyle='87' _moduleid='1343' class='form form1343 formIndex1 formStyle87 fk-formPack ' _createFrom='0' title='' _sys='0' _banId='' style='position:absolute;top:164px;left:-212px;width:198px;' _side='0' _inTab='0' _inMulMCol='0' _inFullmeasure='0' _inpack='0' _inpopupzone='0' _infullmeasurepack='0' _intabpack='0' _inaccordionpack='0' _autoHeight='0' _global='true' _independent='false' data-scrollbar='0'> < class='J_packContent f-packContent f-packContent1343 elemZone elemZoneModule' id='fk-packContent1343'>< class='fk-elemZoneBg J_zoneContentBg elemZoneBg f-packContentBg f-packContentBg1343'> < id='module1336' bannerTitle='文本' _indexClass='' _moduleType='1' _modulestyle='86' _moduleid='1336' class='form form1336 formStyle86 siteEditor ' title='' _sys='0' _banId='' style='position:absolute;top:153px;left:50px;width:113px;' _side='0' _inTab='0' _inMulMCol='0' _inFullmeasure='0' _inpack='1343' _inpopupzone='0' _infullmeasurepack='0' _intabpack='0' _inaccordionpack='0' _autoHeight='0' _global='false' _independent='false' data-scrollbar='0'> < class='lightModuleOuterContent lightModuleOuterContent1336'>< class='fk-editor simpleText '>< style="text-align: center;">---------------------------- < id='module1338' bannerTitle='图片' _indexClass='' _moduleType='1' _modulestyle='79' _moduleid='1338' class='form form1338 formStyle79' title='' _sys='0' _banId='' style='position:absolute;top:34px;left:43px;width:112px;' _side='0' _inTab='0' _inMulMCol='0' _inFullmeasure='0' _inpack='1343' _inpopupzone='0' _infullmeasurepack='0' _intabpack='0' _inaccordionpack='0' _autoHeight='0' _global='false' _independent='false' data-scrollbar='0'> < class='lightModuleOuterContent lightModuleOuterContent1338'>< class='floatImg floatImg_J floatImg_J_special'> < class='floatImgWrap'>< class='forMargin'>< id='float__1338' class=' lazyload_transparent float_in_ J_defImage ' data-original='http://2ms.faisys.com/image/materialLib/other/5000002451.png?v=201712181730' src='//0ms.faisys.com/image/loading/dot.gif' alt='_faiMaterial_5000002451' /> < id='module1339' bannerTitle='图片' _indexClass='' _moduleType='1' _modulestyle='79' _moduleid='1339' class='form form1339 formStyle79 animateModule ' title='' _sys='0' _banId='' style='position:absolute;top:20px;left:125px;width:39px;' _side='0' _inTab='0' _inMulMCol='0' _inFullmeasure='0' _inpack='1343' _inpopupzone='0' _infullmeasurepack='0' _intabpack='0' _inaccordionpack='0' _autoHeight='0' _global='false' _independent='false' data-scrollbar='0'> < class='lightModuleOuterContent lightModuleOuterContent1339'>< class='floatImg floatImg_J floatImg_J_special'> < class='floatImgWrap'>< class='forMargin'>< id='float__1339' class=' lazyload_transparent float_in_ J_defImage ' data-original='http://2ms.faisys.com/image/materialLib/other/5000002446.png?v=201712181730' src='//0ms.faisys.com/image/loading/dot.gif' alt='_faiMaterial_5000002446' /> < id='module1342' _indexClass='' _moduleType='1' _modulestyle='40' _moduleid='1342' class='form form1342 formStyle40' title='' _sys='0' _banId='' style='position:absolute;top:185px;left:39px;width:124px;' _side='0' _inTab='0' _inMulMCol='0' _inFullmeasure='0' _inpack='1343' _inpopupzone='0' _infullmeasurepack='0' _intabpack='0' _inaccordionpack='0' _autoHeight='0' _global='false' _independent='false' data-scrollbar='0'> < class='formTop formTop1342'>< class='left'>< class='center'>< class='right'> < class=' formMiddle formMiddle1342' style='height:156px; 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